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METRNL ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Meteorin like, glial cell differentiation regulator

Gene Aliases

Cometin; meteorin, glial cell differentiation regulator-like; meteorin-like protein; subfatin.

Gene ID

284207

Accession Number

NP_001004431.1

Reactivity

Homo sapiens, Human

Target

Meteorin-like protein

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology METRNL ELISA Kit (Human) (OKEH01814) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for METRNL has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for METRNL is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample METRNL captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

15.6-1000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%

Sensitivity

7.3 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

34 kDa

Specificity

Natural and recombinant Human Meteorin-like protein

Protein Length

311

NCBI Gene Symbol

METRNL

Protein Name

Q641Q3

Gene Name URL

METRNL

Nucleotide Accession Number

NM_001004431.2

Frequently Asked Questions

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