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CES5A ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Carboxylesterase 5A

Gene Aliases

Carboxylesterase 5; carboxylesterase 5A; carboxylesterase 7; carboxylesterase-like urinary excreted protein homolog; CAUXIN; CES4C1; CES5; CES7; epididymis luminal protein 126; HEL126.

Gene ID

221223

Accession Number

NP_001137157.1

Reactivity

Homo sapiens, Human

Target

Carboxylesterase 5A

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology CES5A ELISA Kit (Human) (OKEH01812) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CES5A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CES5A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CES5A captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

78-5000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 104%

Sensitivity

54 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

63 kDa

Specificity

Natural and recombinant Human Carboxylesterase 5A

Protein Length

575

NCBI Gene Symbol

CES5A

Protein Name

Q6NT32

Gene Name URL

CES5A

Nucleotide Accession Number

NM_001143685.1

Frequently Asked Questions

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