HOGA1 ELISA Kit (Human)
Product Specifications
Gene Name
4-hydroxy-2-oxoglutarate aldolase 1
Gene Aliases
4-hydroxy-2-oxoglutarate aldolase, mitochondrial; C10orf65; DHDPS2; DHDPSL; DHDPS-like protein; Dihydrodipicolinate synthase-like; dihydrodipicolinate synthase-like, mitochondrial; dihydrodipicolinate synthetase homolog 2; HP3; N-acetylneuraminate pyruvate lyase 2 (putative) ; NPL2; probable 2-keto-4-hydroxyglutarate aldolase; probable 4-hydroxy-2-oxoglutarate aldolase, mitochondrial; probable KHG-aldolase; protein 569272.
Gene ID
112817
Accession Number
NP_001128142.1
Reactivity
Homo sapiens, Human
Target
4-hydroxy-2-oxoglutarate aldolase, mitochondrial
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology HOGA1 ELISA Kit (Human) (OKEH01807) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HOGA1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HOGA1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HOGA1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
Sensitivity
0.1 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
35 kDa
Specificity
Natural and recombinant Human 4-hydroxy-2-oxoglutarate aldolase, mitochondrial
Protein Length
327
NCBI Gene Symbol
HOGA1
Protein Name
Q86XE5
Gene Name URL
HOGA1
CAS Number
7732-18-5
Nucleotide Accession Number
NM_001134670.1
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