SPINK7 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Serine peptidase inhibitor Kazal type 7
Gene Aliases
ECG2; ECRG2; ECRG-2; esophagus cancer-related gene 2 protein; esophagus cancer-related gene-2; serine peptidase inhibitor, Kazal type 7 (putative) ; serine protease inhibitor Kazal-type 7.
Gene ID
84651
Accession Number
NP_115955.1
Reactivity
Homo sapiens, Human
Target
Serine protease inhibitor Kazal-type 7
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology SPINK7 ELISA Kit (Human) (OKEH01803) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SPINK7 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SPINK7 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SPINK7 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%
Sensitivity
31 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
9 kDa
Specificity
Natural and recombinant Human Serine protease inhibitor Kazal-type 7
Protein Length
85
NCBI Gene Symbol
SPINK7
Protein Name
P58062
Gene Name URL
SPINK7
Nucleotide Accession Number
NM_032566.2
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
BNC471003-100 1x 100 µL
Mucin 5AC (MUC5AC) (58M1), CF647 conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC472853-500 1x 500 µL
CD54 / ICAM-1 (15.2), CF647 conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC470745-100 1x 100 µL
CD45 / LCA (2B11 + PD7/26), CF647 conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC942556-100 1x 100 µL
Spectrin Alpha 1 (Erythrocyte Marker) (rSPTA1/1833), CF594 conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC941219-500 1x 500 µL
Cytokeratin 8 (B22.1), CF594 conjugate, 0.1mg/mL
Sign In for Pricing
View Details