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LETMD1 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

LETM1 domain containing 1

Gene Aliases

1110019O13Rik; cervical cancer 1 proto-oncogene protein p40; cervical cancer proto-oncogene 2 protein; epididymis secretory sperm binding protein; HCCR; HCCR1; HCCR-1; HCCR2; HCCR-2; HCRR-2; human cervical cancer oncogene; LETM1 domain-containing protein 1; regulator of TP53; SLC55A3.

Gene ID

25875

Accession Number

NP_001230618.1

Reactivity

Homo sapiens, Human

Target

LETM1 domain-containing protein 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology LETMD1 ELISA Kit (Human) (OKEH01801) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LETMD1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LETMD1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LETMD1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

31.2-2000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 92%

Sensitivity

16 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

41 kDa

Specificity

Natural and recombinant Human LETM1 domain-containing protein 1

Protein Length

360

NCBI Gene Symbol

LETMD1

Protein Name

Q6P1Q0

Gene Name URL

LETMD1

Nucleotide Accession Number

NM_001243689.1

Frequently Asked Questions

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