LETMD1 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
LETM1 domain containing 1
Gene Aliases
1110019O13Rik; cervical cancer 1 proto-oncogene protein p40; cervical cancer proto-oncogene 2 protein; epididymis secretory sperm binding protein; HCCR; HCCR1; HCCR-1; HCCR2; HCCR-2; HCRR-2; human cervical cancer oncogene; LETM1 domain-containing protein 1; regulator of TP53; SLC55A3.
Gene ID
25875
Accession Number
NP_001230618.1
Reactivity
Homo sapiens, Human
Target
LETM1 domain-containing protein 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology LETMD1 ELISA Kit (Human) (OKEH01801) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LETMD1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LETMD1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LETMD1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
31.2-2000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 92%
Sensitivity
16 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
41 kDa
Specificity
Natural and recombinant Human LETM1 domain-containing protein 1
Protein Length
360
NCBI Gene Symbol
LETMD1
Protein Name
Q6P1Q0
Gene Name URL
LETMD1
Nucleotide Accession Number
NM_001243689.1
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