DEFB119 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Defensin beta 119
Gene Aliases
Beta-defensin 119; Beta-defensin 120; Beta-defensin 19; Beta-defensin 20; DEFB120; DEFB-19; DEFB20; DEFB-20; Defensin, beta 119; defensin, beta 120; defensin, beta 19; defensin, beta 20; ESC42-RELA; ESC42-RELB.
Gene ID
245932
Accession Number
NP_001258138.1
Reactivity
Homo sapiens, Human
Target
Beta-defensin 119
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology DEFB119 ELISA Kit (Human) (OKEH01790) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human DEFB119 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated DEFB119 and incubated. The DEFB119 found in the sample or standards competes with the biotinylated DEFB119 for limited binding sites on the immobilized anti-human DEFB119 antibody. Excess unbound biotinylated DEFB119 and sample or standard DEFB119 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated DEFB119 captured in the well and inversely proportional to the amount of DEFB119 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 84%
Sensitivity
0.12 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
39 kDa
Specificity
Natural and recombinant Human Beta-defensin 119
Protein Length
360
NCBI Gene Symbol
DEFB119
Protein Name
Q8N690
Gene Name URL
DEFB119
Nucleotide Accession Number
NM_001271209.1
Frequently Asked Questions
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