AOX1 ELISA Kit (Bovine)

Product Specifications

CAS Number

7732-18-5

Gene Name

Aldehyde oxidase 1

Gene Aliases

Aldehyde oxidase 1; azaheterocycle hydroxylase 1.

Gene ID

338074

Accession Number

NP_788841.1

Reactivity

Bos taurus, Bovine

Target

Aldehyde oxidase 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology AOX1 ELISA Kit (Bovine) (OKEH01782) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-bovine AOX1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated AOX1 and incubated. The AOX1 found in the sample or standards competes with the biotinylated AOX1 for limited binding sites on the immobilized anti-bovine AOX1 antibody. Excess unbound biotinylated AOX1 and sample or standard AOX1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated AOX1 captured in the well and inversely proportional to the amount of AOX1 which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol