HIST2H2BE ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
H2B clustered histone 21
Gene Aliases
GL105; H2B; H2B histone family, member Q; H2B.1; H2BE; H2BFQ; H2BGL105; H2B-GL105; H2BQ; HIST2H2BE; histone 2, H2be; histone cluster 2 H2B family member e; histone cluster 2, H2be; histone H2B type 2-E; histone H2B.q; histone H2B-GL105.
Gene ID
8349
Accession Number
NP_003519.1
Reactivity
Homo sapiens, Human
Target
Histone H2B type 2-E
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology HIST2H2BE ELISA Kit (Human) (OKEH01774) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HIST2H2BE has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HIST2H2BE is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample HIST2H2BE captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 87%
Sensitivity
0.2 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
13 kDa
Specificity
Natural and recombinant Human Histone H2B type 2-E
Protein Length
126
NCBI Gene Symbol
H2BC21
Protein Name
Q16778
Gene Name URL
H2BC21
Nucleotide Accession Number
NM_003528.2
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