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LPPR1 ELISA Kit (Rat)

Product Specifications

Gene Name

Phospholipid phosphatase related 1

Gene Aliases

Lipid phosphate phosphatase-related protein type 1; Lppr1; phospholipid phosphatase-related protein type 1; plasticity related gene 3; plasticity-related gene 3 protein; plasticity-related protein 3; Prg-3.

Gene ID

298062

Accession Number

NP_958428.1

Reactivity

Rat, Rattus norvegicus

Target

Phospholipid phosphatase-related protein type 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology LPPR1 ELISA Kit (Rat) (OKEH01770) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LPPR1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LPPR1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LPPR1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.78-50ng/mL

Sensitivity

0.39 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

35 kDa

Specificity

Natural and recombinant Rat Lipid phosphate phosphatase-related protein type 1

Protein Length

325

NCBI Gene Symbol

Plppr1

Protein Name

Q6WAY2

Gene Name URL

Plppr1

CAS Number

7732-18-5

Nucleotide Accession Number

NM_201271.1

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