LPPR1 ELISA Kit (Rat)
Product Specifications
Gene Name
Phospholipid phosphatase related 1
Gene Aliases
Lipid phosphate phosphatase-related protein type 1; Lppr1; phospholipid phosphatase-related protein type 1; plasticity related gene 3; plasticity-related gene 3 protein; plasticity-related protein 3; Prg-3.
Gene ID
298062
Accession Number
NP_958428.1
Reactivity
Rat, Rattus norvegicus
Target
Phospholipid phosphatase-related protein type 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology LPPR1 ELISA Kit (Rat) (OKEH01770) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LPPR1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LPPR1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LPPR1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.78-50ng/mL
Sensitivity
0.39 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
35 kDa
Specificity
Natural and recombinant Rat Lipid phosphate phosphatase-related protein type 1
Protein Length
325
NCBI Gene Symbol
Plppr1
Protein Name
Q6WAY2
Gene Name URL
Plppr1
CAS Number
7732-18-5
Nucleotide Accession Number
NM_201271.1
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