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LILRB4 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Leukocyte immunoglobulin like receptor B4

Gene Aliases

CD85 antigen-like family member K; CD85K; ILT3; ILT-3; immunoglobulin-like transcript 3; leucocyte Ig-like receptor B4; leukocyte immunoglobulin-like receptor 5; leukocyte immunoglobulin-like receptor subfamily B member 4; leukocyte immunoglobulin-like receptor, subfamily B (with TM and ITIM domains), member 4; LIR5; LIR-5; monocyte inhibitory receptor HM18.

Gene ID

11006

Accession Number

NP_001265355.2

Reactivity

Homo sapiens, Human

Target

Leukocyte immunoglobulin-like receptor subfamily B member 4

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology LILRB4 ELISA Kit (Human) (OKEH01767) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LILRB4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LILRB4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LILRB4 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%

Sensitivity

0.082 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

49 kDa

Specificity

Natural and recombinant Human Leukocyte immunoglobulin-like receptor subfamily B member 4

Protein Length

448

NCBI Gene Symbol

LILRB4

Protein Name

Q8NHJ6

Gene Name URL

LILRB4

Nucleotide Accession Number

NM_001278426.3

Frequently Asked Questions

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