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TBX21 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

T-box transcription factor 21

Gene Aliases

T-bet; TBET; TBLYM; T-box 21; T-box expressed in T cells; T-box protein 21; T-box transcription factor TBX21; T-cell-specific T-box transcription factor T-bet; T-PET; transcription factor TBLYM.

Gene ID

30009

Accession Number

NP_037483.1

Reactivity

Homo sapiens, Human

Target

T-box transcription factor TBX21

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology TBX21 ELISA Kit (Human) (OKEH01759) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TBX21 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TBX21 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TBX21 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 115%

Sensitivity

0.098 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

58 kDa

Specificity

Natural and recombinant Human T-box transcription factor TBX21

Protein Length

535

NCBI Gene Symbol

TBX21

Protein Name

Q9UL17

Gene Name URL

TBX21

Nucleotide Accession Number

NM_013351.1

Frequently Asked Questions

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