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TCF7L2 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Transcription factor 7 like 2

Gene Aliases

HMG box transcription factor 4; hTCF-4; T-cell factor 4; T-cell-specific transcription factor 4; TCF4; TCF-4; transcription factor 7-like 2; transcription factor 7-like 2 (T-cell specific, HMG-box) .

Gene ID

6934

Accession Number

NP_001139746.1

Reactivity

Homo sapiens, Human

Target

Transcription factor 7-like 2

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology TCF7L2 ELISA Kit (Human) (OKEH01758) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TCF7L2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TCF7L2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TCF7L2 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

78-5000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 106%

Sensitivity

54 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

68 kDa

Specificity

Natural and recombinant Human Transcription factor 7-like 2

Protein Length

619

NCBI Gene Symbol

TCF7L2

Protein Name

Q9NQB0

Gene Name URL

TCF7L2

Nucleotide Accession Number

NM_001146274.1

Frequently Asked Questions

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