TCF7L2 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Transcription factor 7 like 2
Gene Aliases
HMG box transcription factor 4; hTCF-4; T-cell factor 4; T-cell-specific transcription factor 4; TCF4; TCF-4; transcription factor 7-like 2; transcription factor 7-like 2 (T-cell specific, HMG-box) .
Gene ID
6934
Accession Number
NP_001139746.1
Reactivity
Homo sapiens, Human
Target
Transcription factor 7-like 2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology TCF7L2 ELISA Kit (Human) (OKEH01758) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TCF7L2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TCF7L2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TCF7L2 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 106%
Sensitivity
54 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
68 kDa
Specificity
Natural and recombinant Human Transcription factor 7-like 2
Protein Length
619
NCBI Gene Symbol
TCF7L2
Protein Name
Q9NQB0
Gene Name URL
TCF7L2
Nucleotide Accession Number
NM_001146274.1
Frequently Asked Questions
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