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TTF2 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Transcription termination factor 2

Gene Aliases

F2; HuF2; human factor 2; lodestar homolog; lodestar protein; RNA polymerase II termination factor; transcription release factor 2; transcription termination factor 2; transcription termination factor, RNA polymerase II; ZGRF6; zinc finger, GRF-type containing 6.

Gene ID

8458

Accession Number

NP_003585.3

Reactivity

Homo sapiens, Human

Target

Transcription termination factor 2

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology TTF2 ELISA Kit (Human) (OKEH01757) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TTF2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TTF2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TTF2 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Urine, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

78-5000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 108%

Sensitivity

56 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

129 kDa

Specificity

Natural and recombinant Human Transcription termination factor 2

Protein Length

1162

NCBI Gene Symbol

TTF2

Protein Name

Q9UNY4

Gene Name URL

TTF2

Nucleotide Accession Number

NM_003594.3

Frequently Asked Questions

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