ZFP36 ELISA Kit (Human)
Selected Variant
OKEH01753-96W
Size: 96 Tests
Product Specifications
CAS Number
7732-18-5
Gene Name
ZFP36 ring finger protein
Gene Aliases
G0/G1 switch regulatory protein 24; G0S24; GOS24; growth factor-inducible nuclear protein NUP475; mRNA decay activator protein ZFP36; NUP475; RNF162A; TIS11; tristetraprolin; tristetraproline; TTP; zfp-36; Zinc finger protein 36; zinc finger protein 36 homolog; zinc finger protein 36, C3H type, homolog.
Gene ID
7538
Accession Number
NP_003398.2
Reactivity
Homo sapiens, Human
Target
MRNA decay activator protein ZFP36
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology ZFP36 ELISA Kit (Human) (OKEH01753) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ZFP36 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ZFP36 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ZFP36 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
31.2-2000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 89%
Sensitivity
14 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
34 kDa
Specificity
Natural and recombinant Human mRNA decay activator protein ZFP36
Protein Length
326
NCBI Gene Symbol
ZFP36
Protein Name
P26651
Gene Name URL
ZFP36
Nucleotide Accession Number
NM_003407.3
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