FEM1A ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Fem-1 homolog A
Gene Aliases
EPRAP; FEM1-alpha; prostaglandin E receptor 4-associated protein; protein fem-1 homolog A.
Gene ID
55527
Accession Number
NP_061178.1
Reactivity
Homo sapiens, Human
Target
Protein fem-1 homolog A
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology FEM1A ELISA Kit (Human) (OKEH01739) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for FEM1A has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for FEM1A is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample FEM1A captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 86%
Sensitivity
0.16 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
73 kDa
Specificity
Natural and recombinant Human Protein fem-1 homolog A
Protein Length
669
NCBI Gene Symbol
FEM1A
Protein Name
Q9BSK4
Gene Name URL
FEM1A
Nucleotide Accession Number
NM_018708.2
Frequently Asked Questions
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