TIRAP ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
TIR domain containing adaptor protein
Gene Aliases
Adapter protein wyatt; adaptor protein Wyatt; BACTS1; Mal; MyD88 adapter-like protein; MyD88-2; toll/interleukin-1 receptor domain-containing adapter protein; toll-interleukin 1 receptor (TIR) domain containing adaptor protein; Toll-like receptor adaptor protein; wyatt.
Gene ID
114609
Accession Number
NP_001034750.1
Reactivity
Homo sapiens, Human
Target
Toll/interleukin-1 receptor domain-containing adapter protein
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology TIRAP ELISA Kit (Human) (OKEH01734) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TIRAP has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TIRAP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TIRAP captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
31.2-2000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%
Sensitivity
15 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
24 kDa
Specificity
Natural and recombinant Human Toll/interleukin-1 receptor domain-containing adapter protein
Protein Length
221
NCBI Gene Symbol
TIRAP
Protein Name
P58753
Gene Name URL
TIRAP
Nucleotide Accession Number
NM_001039661.1
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
BNC680177-100 1x 100 µL
CD50 (ICAM-3) (101-1D2), CF568 conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC400012-100 1x 100 µL
Tyrosinase (T311), CF640R conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC680218-100 1x 100 µL
CD100 (Semaphorin-4D) (A8), CF568 conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC470177-100 1x 100 µL
CD50 (ICAM-3) (101-1D2), CF647 conjugate, 0.1mg/mL
Sign In for Pricing
View Details 
BNC400031-500 1x 500 µL
Mucin 5AC (MUC5AC) (45M1), CF640R conjugate, 0.1mg/mL
Sign In for Pricing
View Details