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RSAD2 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Radical S-adenosyl methionine domain containing 2

Gene Aliases

Cig33; cig5; cytomegalovirus-induced gene 5 protein; radical S-adenosyl methionine domain-containing protein 2; vig1; viperin; virus inhibitory protein, endoplasmic reticulum-associated, interferon-inducible.

Gene ID

91543

Accession Number

NP_542388.2

Reactivity

Homo sapiens, Human

Target

Radical S-adenosyl methionine domain-containing protein 2

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology RSAD2 ELISA Kit (Human) (OKEH01720) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for RSAD2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for RSAD2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample RSAD2 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.78-50ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 89%

Sensitivity

0.072 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

42 kDa

Specificity

Natural and recombinant Human Radical S-adenosyl methionine domain-containing protein 2

Protein Length

361

NCBI Gene Symbol

RSAD2

Protein Name

Q8WXG1

Gene Name URL

RSAD2

Nucleotide Accession Number

NM_080657.4

Frequently Asked Questions

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