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TBPL1 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

TATA-box binding protein like 1

Gene Aliases

21 kDa TBP-like protein;21-kDA TBP-like protein; MGC:8389; MGC:9620; second TBP of unique DNA protein; STUD; TATA box-binding protein-like 1; TATA box-binding protein-like protein 1; TATA box-binding protein-related factor 2; TBP-like 1; TBP-like factor; TBP-related factor 2; TBP-related protein; TLF; TLP; TRF2.

Gene ID

9519

Accession Number

NP_001240605.1

Reactivity

Homo sapiens, Human

Target

TATA box-binding protein-like protein 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology TBPL1 ELISA Kit (Human) (OKEH01713) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TBPL1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TBPL1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TBPL1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

78-5000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 107%

Sensitivity

39 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

20 kDa

Specificity

Natural and recombinant Human TATA box-binding protein-like protein 1

Protein Length

186

NCBI Gene Symbol

TBPL1

Protein Name

P62380

Gene Name URL

TBPL1

Nucleotide Accession Number

NM_001253676.1

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