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TARBP2 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

TARBP2 subunit of RISC loading complex

Gene Aliases

LOQS; RISC-loading complex subunit TARBP2; TAR (HIV) RNA-binding protein 2; TAR (HIV) RNA-binding protein TRBP1; TAR (HIV-1) RNA binding protein 2; TAR RNA binding protein 2; TAR RNA-binding protein 2; TARBP2, RISC loading complex RNA binding subunit; trans-activation responsive RNA-binding protein; Trans-activation-responsive RNA-binding protein; TRBP; TRBP1; TRBP2.

Gene ID

6895

Accession Number

NP_004169.3

Reactivity

Homo sapiens, Human

Target

RISC-loading complex subunit TARBP2

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology TARBP2 ELISA Kit (Human) (OKEH01711) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TARBP2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TARBP2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TARBP2 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.312-20ng/ml

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 114%

Sensitivity

0.19 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

39 kDa

Specificity

Natural and recombinant Human RISC-loading complex subunit TARBP2

Protein Length

366

NCBI Gene Symbol

TARBP2

Protein Name

Q15633

Gene Name URL

TARBP2

Nucleotide Accession Number

NM_004178.4

Frequently Asked Questions

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