TARBP2 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
TARBP2 subunit of RISC loading complex
Gene Aliases
LOQS; RISC-loading complex subunit TARBP2; TAR (HIV) RNA-binding protein 2; TAR (HIV) RNA-binding protein TRBP1; TAR (HIV-1) RNA binding protein 2; TAR RNA binding protein 2; TAR RNA-binding protein 2; TARBP2, RISC loading complex RNA binding subunit; trans-activation responsive RNA-binding protein; Trans-activation-responsive RNA-binding protein; TRBP; TRBP1; TRBP2.
Gene ID
6895
Accession Number
NP_004169.3
Reactivity
Homo sapiens, Human
Target
RISC-loading complex subunit TARBP2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology TARBP2 ELISA Kit (Human) (OKEH01711) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TARBP2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TARBP2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TARBP2 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 114%
Sensitivity
0.19 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
39 kDa
Specificity
Natural and recombinant Human RISC-loading complex subunit TARBP2
Protein Length
366
NCBI Gene Symbol
TARBP2
Protein Name
Q15633
Gene Name URL
TARBP2
Nucleotide Accession Number
NM_004178.4
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