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SIK2 ELISA Kit (Human)

Selected Variant

OKEH01706-96W

Size: 96 Well

Product Specifications

CAS Number

7732-18-5

Gene Name

Salt inducible kinase 2

Gene Aliases

LOH11CR1I; QIK; qin-induced kinase; Salt-inducible kinase 2; salt-inducible protein kinase 2; salt-inducible serine/threonine kinase 2; serine/threonine-protein kinase SIK2; serine/threonine-protein kinase SNF1-like kinase 2; SIK-2; SNF1-like kinase 2; SNF1LK2.

Gene ID

23235

Accession Number

NP_056006.1

Reactivity

Homo sapiens, Human

Target

Serine/threonine-protein kinase SIK2

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology SIK2 ELISA Kit (Human) (OKEH01706) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human SIK2 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated SIK2 and incubated. The SIK2 found in the sample or standards competes with the biotinylated SIK2 for limited binding sites on the immobilized anti-human SIK2 antibody. Excess unbound biotinylated SIK2 and sample or standard SIK2 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated SIK2 captured in the well and inversely proportional to the amount of SIK2 which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.312-20ng/ml

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 93%

Sensitivity

0.15 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

103 kDa

Specificity

Natural and recombinant Human Serine/threonine-protein kinase SIK2

Protein Length

926

NCBI Gene Symbol

SIK2

Protein Name

Q9H0K1

Gene Name URL

SIK2

Nucleotide Accession Number

NM_015191.2

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