RUVBL1 ELISA Kit (Human)

49 kDa TATA box-binding protein-interacting protein;49 kDa TBP-interacting protein;54 kDa erythrocyte cytosolic protein; ECP54; ECP-54; epididymis secretory sperm binding protein; INO80 complex subunit H; INO80H; NMP 238; NMP238; nuclear matrix protein 238; PONTIN; pontin 52; Pontin52; RuvB (E coli homolog) -like 1; ruvB-like 1; RuvB-like AAA ATPase; RVB1; TAP54-alpha; TATA binding protein interacting protein 49 kDa; TIH1; TIP49; TIP49A; TIP60-associated protein 54-alpha.

Aviva Systems Biology RUVBL1 ELISA Kit (Human) (OKEH01702) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for RUVBL1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for RUVBL1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample RUVBL1 captured in well.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol