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RSPO1 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

R-spondin 1

Gene Aliases

CRISTIN3; roof plate-specific spondin-1; RSPO; R-spondin homolog; R-spondin-1.

Gene ID

284654

Accession Number

NP_001033722.1

Reactivity

Homo sapiens, Human

Target

R-spondin-1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology RSPO1 ELISA Kit (Human) (OKEH01701) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for RSPO1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for RSPO1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample RSPO1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

78-5000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%

Sensitivity

43 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

29 kDa

Specificity

Natural and recombinant Human R-spondin-1

Protein Length

263

NCBI Gene Symbol

RSPO1

Protein Name

Q2MKA7

Gene Name URL

RSPO1

Nucleotide Accession Number

NM_001038633.3

Frequently Asked Questions

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