TAB2 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
TGF-beta activated kinase 1 (MAP3K7) binding protein 2
Gene Aliases
CHTD2; MAP3K7IP2; mitogen-activated protein kinase kinase kinase 7-interacting protein 2; TAB-2; TAK1-binding protein 2; TGF-beta-activated kinase 1 and MAP3K7-binding protein 2; TGF-beta-activated kinase 1-binding protein 2.
Gene ID
23118
Accession Number
NP_001278963.1
Reactivity
Homo sapiens, Human
Target
TGF-beta-activated kinase 1 and MAP3K7-binding protein 2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology TAB2 ELISA Kit (Human) (OKEH01678) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TAB2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TAB2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TAB2 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
15.6-1000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 87%
Sensitivity
10 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
76 kDa
Specificity
Natural and recombinant Human TGF-beta-activated kinase 1 and MAP3K7-binding protein 2
Protein Length
693
NCBI Gene Symbol
TAB2
Protein Name
Q9NYJ8
Gene Name URL
TAB2
Nucleotide Accession Number
NM_001292034.2
Frequently Asked Questions
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