What is the catalog number for CLPP ELISA Kit (Human)?

The catalog number for CLPP ELISA Kit (Human) is OKEH01658-96W.

What sizes are available for CLPP ELISA Kit (Human)?

CLPP ELISA Kit (Human) is available in the following size(s): 96 Well.

Is CLPP ELISA Kit (Human) in stock?

CLPP ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

What is the purity of this recombinant protein?

Purity, typically assessed by SDS-PAGE, is listed on the datasheet under quality control data.

What do I do if this product arrives with reduced ice or a damaged cold-chain package?

Report this to us immediately with photos and your order reference. Our specialists will assess whether the product is still usable given the cold-chain interruption, and will arrange a replacement or credit if it isn’t.

What host species was used to raise this antibody?

The host species used in production is listed under product specifications on the datasheet, and is relevant when selecting a compatible secondary antibody for your assay.

CLPP ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Caseinolytic mitochondrial matrix peptidase proteolytic subunit

Gene Aliases

ATP-dependent Clp protease proteolytic subunit, mitochondrial; ATP-dependent protease ClpAP, proteolytic subunit, human; ClpP caseinolytic peptidase ATP-dependent, proteolytic subunit; ClpP caseinolytic peptidase, ATP-dependent, proteolytic subunit homolog; ClpP caseinolytic protease, ATP-dependent, proteolytic subunit homolog; DFNB81; endopeptidase Clp; PRLTS3; putative ATP-dependent Clp protease proteolytic subunit, mitochondrial.

Gene ID

8192

Accession Number

NP_006003.1

Reactivity

Homo sapiens, Human

Target

ATP-dependent Clp protease proteolytic subunit, mitochondrial

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology CLPP ELISA Kit (Human) (OKEH01658) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human CLPP antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated CLPP and incubated. The CLPP found in the sample or standards competes with the biotinylated CLPP for limited binding sites on the immobilized anti-human CLPP antibody. Excess unbound biotinylated CLPP and sample or standard CLPP is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated CLPP captured in the well and inversely proportional to the amount of CLPP which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol