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AKR1C4 ELISA Kit (Human)

Selected Variant

OKEH01649-96W

Size: 96 Well

Product Specifications

CAS Number

7732-18-5

Gene Name

Aldo-keto reductase family 1 member C4

Gene Aliases

3-alpha-HSD;3-alpha-HSD1;3alpha-hydroxysteroid 3-dehydrogenase;3-alpha-hydroxysteroid dehydrogenase type I; aldo-keto reductase family 1 member C4; C11; CDR; CHDR; Chlordecone reductase; chlordecone reductase; 3-alpha hydroxysteroid dehydrogenase, type I; dihydrodiol dehydrogenase 4; DD4; DD-4; Dihydrodiol dehydrogenase 4; dihydrodiol dehydrogenase isozyme DD4; HAKRA; type I 3-alpha-hydroxysteroid dehydrogenase.

Gene ID

1109

Accession Number

NP_001809.3

Reactivity

Homo sapiens, Human

Target

Aldo-keto reductase family 1 member C4

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology AKR1C4 ELISA Kit (Human) (OKEH01649) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for AKR1C4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for AKR1C4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample AKR1C4 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 1%

Sensitivity

0.078 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

37 kDa

Specificity

Natural and recombinant Human Aldo-keto reductase family 1 member C4

Protein Length

323

NCBI Gene Symbol

AKR1C4

Protein Name

P17516

Gene Name URL

AKR1C4

Nucleotide Accession Number

NM_001818.3

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