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PDCD4 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Programmed cell death 4

Gene Aliases

H731; neoplastic transformation inhibitor protein; nuclear antigen H731; Nuclear antigen H731-like; programmed cell death 4 (neoplastic transformation inhibitor) ; programmed cell death protein 4; protein 197/15a.

Gene ID

27250

Accession Number

NP_055271.2

Reactivity

Homo sapiens, Human

Target

Programmed cell death protein 4

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology PDCD4 ELISA Kit (Human) (OKEH01644) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PDCD4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PDCD4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PDCD4 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

0.312-20ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%

Sensitivity

0.13 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

52 kDa

Specificity

Natural and recombinant Human Programmed cell death protein 4

Protein Length

469

NCBI Gene Symbol

PDCD4

Protein Name

Q53EL6

Gene Name URL

PDCD4

Nucleotide Accession Number

NM_014456.4

Frequently Asked Questions

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