What is the catalog number for HUWE1 ELISA Kit (Rat)?

The catalog number for HUWE1 ELISA Kit (Rat) is OKEH01643-96W.

What sizes are available for HUWE1 ELISA Kit (Rat)?

HUWE1 ELISA Kit (Rat) is available in the following size(s): 96 Well.

Is HUWE1 ELISA Kit (Rat) in stock?

HUWE1 ELISA Kit (Rat) is currently made to order / not held in immediate stock. Contact us for lead time.

Is this kit compatible with automated liquid handling platforms?

Automation compatibility varies by kit and isn’t always listed on the datasheet. Contact our technical support team with details of your platform so we can confirm with the manufacturer.

What applications has this product been validated for?

Validated applications such as WB, ELISA, IHC, ICC, or FC are listed on the product datasheet. If you need application-specific validation data before ordering, contact our technical support team with the catalogue number.

How do I reconstitute this lyophilized product?

Reconstitution instructions, including the recommended diluent and final concentration, are provided on the datasheet. Aliquot immediately after reconstitution to avoid repeated freeze-thaw cycles.

HUWE1 ELISA Kit (Rat)

Product Specifications

CAS Number

7732-18-5

Gene Name

HECT, UBA and WWE domain containing 1, E3 ubiquitin protein ligase

Gene Aliases

HECT, UBA and WWE domain-containing protein 1; HECT-type E3 ubiquitin transferase HUWE1; Upstream regulatory element-binding protein 1.

Gene ID

501546

Reactivity

Rat, Rattus norvegicus

Target

E3 ubiquitin-protein ligase HUWE1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology HUWE1 ELISA Kit (Rat) (OKEH01643) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-rat HUWE1 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated HUWE1 and incubated. The HUWE1 found in the sample or standards competes with the biotinylated HUWE1 for limited binding sites on the immobilized anti-rat HUWE1 antibody. Excess unbound biotinylated HUWE1 and sample or standard HUWE1 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated HUWE1 captured in the well and inversely proportional to the amount of HUWE1 which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol