KCNB2 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Potassium voltage-gated channel subfamily B member 2
Gene Aliases
Delayed rectifier potassium channel protein; KV2.2; potassium channel Kv2.2; potassium channel, voltage gated Shab related subfamily B, member 2; potassium voltage-gated channel subfamily B member 2; potassium voltage-gated channel, Shab-related subfamily, member 2; voltage-gated potassium channel subunit Kv2.2.
Gene ID
9312
Accession Number
NP_004761.2
Reactivity
Homo sapiens, Human
Target
Potassium voltage-gated channel subfamily B member 2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology KCNB2 ELISA Kit (Human) (OKEH01638) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human KCNB2 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated KCNB2 and incubated. The KCNB2 found in the sample or standards competes with the biotinylated KCNB2 for limited binding sites on the immobilized anti-human KCNB2 antibody. Excess unbound biotinylated KCNB2 and sample or standard KCNB2 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated KCNB2 captured in the well and inversely proportional to the amount of KCNB2 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 98%
Sensitivity
0.13 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
102 kDa
Specificity
Natural and recombinant Human Potassium voltage-gated channel subfamily B member 2
Protein Length
911
NCBI Gene Symbol
KCNB2
Protein Name
Q92953
Gene Name URL
KCNB2
Nucleotide Accession Number
NM_004770.2
Frequently Asked Questions
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