PMEL ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Premelanosome protein
Gene Aliases
D12S53E; gp100; ME20; ME20M; ME20-M; melanocyte protein mel 17; melanocyte protein PMEL; melanocyte protein Pmel 17; melanocytes lineage-specific antigen GP100; melanoma-associated ME20 antigen; melanosomal matrix protein17; P1; P100; PMEL17; Premelanosome protein; SI; SIL; SILV; silver locus protein homolog; silver, mouse, homolog of.
Gene ID
6490
Accession Number
NP_001186982.1
Reactivity
Homo sapiens, Human
Target
Melanocyte protein PMEL
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology PMEL ELISA Kit (Human) (OKEH01630) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PMEL has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PMEL is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PMEL captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
0.156-10ng/mL
Sensitivity
0.078 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
70 kDa
Specificity
Natural and recombinant Human Melanocyte protein PMEL
Protein Length
661
NCBI Gene Symbol
PMEL
Protein Name
P40967
Gene Name URL
PMEL
Nucleotide Accession Number
NM_001200053.1
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