KRT10 ELISA Kit (Dog)
Product Specifications
CAS Number
7732-18-5
Gene Aliases
Cytokeratin-10; Epithelial keratin-10; Keratin-10.
Gene ID
491006
Accession Number
XP_013972063.1
Reactivity
Canis lupus, Dog
Target
Keratin, type I cytoskeletal 10
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Competitive
Assay Principle
Aviva Systems Biology KRT10 ELISA Kit (Dog) (OKEH01622) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-dog KRT10 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated KRT10 and incubated. The KRT10 found in the sample or standards competes with the biotinylated KRT10 for limited binding sites on the immobilized anti-dog KRT10 antibody. Excess unbound biotinylated KRT10 and sample or standard KRT10 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated KRT10 captured in the well and inversely proportional to the amount of KRT10 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101%
Sensitivity
0.099 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
57 kDa
Specificity
Natural and recombinant Dog Keratin, type I cytoskeletal 10
Protein Length
568
NCBI Gene Symbol
KRT10
Protein Name
Q6EIZ0
Gene Name URL
KRT10
Nucleotide Accession Number
XM_014116588.1
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