What is the catalog number for NEFH ELISA Kit (Human)?

The catalog number for NEFH ELISA Kit (Human) is OKEH01605-96W.

What sizes are available for NEFH ELISA Kit (Human)?

NEFH ELISA Kit (Human) is available in the following size(s): 96 Well.

Is NEFH ELISA Kit (Human) in stock?

NEFH ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

Can I return this product if I ordered the wrong one?

As a consumer, you have a 14-day right of withdrawal from receipt under EU consumer protection law, submitted via our withdrawal/cancellation request form. Note this applies to unused, undamaged products, and doesn’t override the expiry/storage conditions on performance-based claims.

Is this product tested for endotoxin levels?

Endotoxin testing status is noted on the datasheet where applicable, particularly for recombinant proteins intended for cell-based or functional assays. If this isn’t listed and you need confirmation, contact support with the catalogue number.

Can I request additional validation data, such as western blot images, for this product?

Additional data may be available on request. Contact our technical support team with the catalogue number and we’ll check what supporting documentation the supplier can provide.

NEFH ELISA Kit (Human)

Selected Variant

OKEH01605-96W

Size: 96 Well

Product Specifications

CAS Number

7732-18-5

Gene Name

Neurofilament heavy chain

Gene Aliases

200 kDa neurofilament protein; CMT2CC; neurofilament heavy polypeptide; neurofilament triplet H protein; neurofilament, heavy polypeptide 200kDa; NFH; NF-H.

Gene ID

4744

Accession Number

NP_066554.2

Reactivity

Homo sapiens, Human

Target

Neurofilament heavy polypeptide

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology NEFH ELISA Kit (Human) (OKEH01605) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NEFH has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NEFH is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NEFH captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

3.12-200pg/mL

Recovery

<= 3.6%

Sensitivity

0.78 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

112 kDa

Specificity

Natural and recombinant Human Neurofilament heavy polypeptide

Protein Length

1026

NCBI Gene Symbol

NEFH

Protein Name

P12036

Gene Name URL

NEFH

Nucleotide Accession Number

NM_021076.3

Price

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