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SOD2 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Superoxide dismutase 2

Gene Aliases

Epididymis secretory sperm binding protein; gastric cancer-associated lncRNA 1; GClnc1; indophenoloxidase B; IPOB; IPO-B; manganese-containing superoxide dismutase; mangano-superoxide dismutase; Mn superoxide dismutase; Mn-SOD; MNSOD; MVCD6; superoxide dismutase [Mn], mitochondrial; superoxide dismutase 2, mitochondrial.

Gene ID

6648

Accession Number

NP_000627.2

Reactivity

Homo sapiens, Human

Target

Superoxide dismutase [Mn], mitochondrial

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Competitive

Assay Principle

Aviva Systems Biology SOD2 ELISA Kit (Human) (OKEH01567) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SOD2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SOD2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SOD2 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

31.2-2000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 93%

Sensitivity

12 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

25 kDa

Specificity

Natural and recombinant Human Superoxide dismutase [Mn], mitochondrial

Protein Length

222

NCBI Gene Symbol

SOD2

Protein Name

P04179

Gene Name URL

SOD2

Nucleotide Accession Number

NM_000636.2

Frequently Asked Questions

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