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SOD3 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Superoxide dismutase 3

Gene Aliases

EC-SOD; extracellular superoxide dismutase [Cu-Zn]; superoxide dismutase 3, extracellular; testicular tissue protein Li 175.

Gene ID

6649

Accession Number

NP_003093.2

Reactivity

Homo sapiens, Human

Target

Extracellular superoxide dismutase [Cu-Zn]

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology SOD3 ELISA Kit (Human) (OKEH01559) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human SOD3 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated SOD3 and incubated. The SOD3 found in the sample or standards competes with the biotinylated SOD3 for limited binding sites on the immobilized anti-human SOD3 antibody. Excess unbound biotinylated SOD3 and sample or standard SOD3 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated SOD3 captured in the well and inversely proportional to the amount of SOD3 which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

0.312-20ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 105%

Sensitivity

0.103 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

26 kDa

Specificity

Natural and recombinant Human Extracellular superoxide dismutase [Cu-Zn]

Protein Length

240

NCBI Gene Symbol

SOD3

Protein Name

P08294

Gene Name URL

SOD3

Nucleotide Accession Number

NM_003102.2

Frequently Asked Questions

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