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ZG16 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Zymogen granule protein 16

Gene Aliases

Jacalin-like lectin domain containing; JCLN; JCLN1; secretory lectin ZG16; ZG16A; zymogen granule membrane protein 16; zymogen granule protein 16 homolog.

Gene ID

653808

Accession Number

NP_689551.2

Reactivity

Homo sapiens, Human

Target

Zymogen granule membrane protein 16

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology ZG16 ELISA Kit (Human) (OKEH01554) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human ZG16 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated ZG16 and incubated. The ZG16 found in the sample or standards competes with the biotinylated ZG16 for limited binding sites on the immobilized anti-human ZG16 antibody. Excess unbound biotinylated ZG16 and sample or standard ZG16 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated ZG16 captured in the well and inversely proportional to the amount of ZG16 which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids

Detection Range

0.156-10ng/ml

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 120%

Sensitivity

0.078 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

18 kDa

Specificity

Natural and recombinant Human Zymogen granule membrane protein 16

Protein Length

167

NCBI Gene Symbol

ZG16

Protein Name

O60844

Gene Name URL

ZG16

Nucleotide Accession Number

NM_152338.3

Frequently Asked Questions

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