ZG16 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Zymogen granule protein 16
Gene Aliases
Jacalin-like lectin domain containing; JCLN; JCLN1; secretory lectin ZG16; ZG16A; zymogen granule membrane protein 16; zymogen granule protein 16 homolog.
Gene ID
653808
Accession Number
NP_689551.2
Reactivity
Homo sapiens, Human
Target
Zymogen granule membrane protein 16
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology ZG16 ELISA Kit (Human) (OKEH01554) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human ZG16 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated ZG16 and incubated. The ZG16 found in the sample or standards competes with the biotinylated ZG16 for limited binding sites on the immobilized anti-human ZG16 antibody. Excess unbound biotinylated ZG16 and sample or standard ZG16 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated ZG16 captured in the well and inversely proportional to the amount of ZG16 which was contained in the sample or standard.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Detection Range
0.156-10ng/ml
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 120%
Sensitivity
0.078 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
18 kDa
Specificity
Natural and recombinant Human Zymogen granule membrane protein 16
Protein Length
167
NCBI Gene Symbol
ZG16
Protein Name
O60844
Gene Name URL
ZG16
Nucleotide Accession Number
NM_152338.3
Frequently Asked Questions
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