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CSPG4 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Chondroitin sulfate proteoglycan 4

Gene Aliases

Chondroitin sulfate proteoglycan 4; chondroitin sulfate proteoglycan 4 (melanoma-associated) ; chondroitin sulfate proteoglycan NG2; CSPG4A; HMW-MAA; MCSP; MCSPG; melanoma chondroitin sulfate proteoglycan; melanoma-associated chondroitin sulfate proteoglycan; MEL-CSPG; MSK16; NG2.

Gene ID

1464

Accession Number

NP_001888.2

Reactivity

Homo sapiens, Human

Target

Chondroitin sulfate proteoglycan 4

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology CSPG4 ELISA Kit (Human) (OKEH01517) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CSPG4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CSPG4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample CSPG4 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 93%

Sensitivity

0.078 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

251 kDa

Specificity

Natural and recombinant Human Chondroitin sulfate proteoglycan 4

Protein Length

2322

NCBI Gene Symbol

CSPG4

Protein Name

Q6UVK1

Gene Name URL

CSPG4

Nucleotide Accession Number

NM_001897.4

Frequently Asked Questions

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