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NPR1 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Natriuretic peptide receptor 1

Gene Aliases

ANPa; ANP-A; ANPRA; ANPR-A; atrial natriuretic peptide receptor 1; atrial natriuretic peptide receptor type A; atrionatriuretic peptide receptor A; GC-A; guanylate cyclase A; GUC2A; GUCY2A; natriuretic peptide A type receptor; natriuretic peptide receptor A; natriuretic peptide receptor A/guanylate cyclase A (atrionatriuretic peptide receptor A) ; NPRA; NPR-A; testicular tissue protein Li 20.

Gene ID

4881

Accession Number

NP_000897.3

Reactivity

Homo sapiens, Human

Target

Atrial natriuretic peptide receptor 1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology NPR1 ELISA Kit (Human) (OKEH01512) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NPR1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NPR1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NPR1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

0.312-20ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 93%

Sensitivity

0.15 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

119 kDa

Specificity

Natural and recombinant Human Atrial natriuretic peptide receptor 1

Protein Length

1061

NCBI Gene Symbol

NPR1

Protein Name

P16066

Gene Name URL

NPR1

Nucleotide Accession Number

NM_000906.3

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