Welcome to GenPrice! Check out our latest updates.

Shopping Cart (0)

Your cart is empty

Add some products to get started!

TK1 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Thymidine kinase 1

Gene Aliases

Cytosolic thymidine kinase; epididymis secretory sperm binding protein; thymidine kinase, cytosolic; TK2.

Gene ID

7083

Accession Number

NP_003249.3

Reactivity

Homo sapiens, Human

Target

Thymidine kinase, cytosolic

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology TK1 ELISA Kit (Human) (OKEH01499) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TK1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TK1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample TK1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

0.06-4pmol/L

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 96%

Sensitivity

0.01pmol/L

Reconstitution

Store as indicated in product manual.

Molecular Weight

25 kDa

Specificity

Natural and recombinant Human Thymidine kinase, cytosolic

Protein Length

234

NCBI Gene Symbol

TK1

Protein Name

P04183

Gene Name URL

TK1

Nucleotide Accession Number

NM_003258.4

Frequently Asked Questions

More Discoveries

Explore Other Products

Browse additional items from our catalog

CD11a (Cris-3), CF405S conjugate, 0.1mg/mL
BNC040151-100 1x 100 µL

CD11a (Cris-3), CF405S conjugate, 0.1mg/mL

Sign In for Pricing
View Details
CD45 / LCA (2B11 + PD7/26), CF405S conjugate, 0.1mg/mL
BNC040745-500 1x 500 µL

CD45 / LCA (2B11 + PD7/26), CF405S conjugate, 0.1mg/mL

Sign In for Pricing
View Details
CD74 (BU45), CF568 conjugate, 0.1mg/mL
BNC680189-500 1x 500 µL

CD74 (BU45), CF568 conjugate, 0.1mg/mL

Sign In for Pricing
View Details
CD3e (CRIS-7), CF640R conjugate, 0.1mg/mL
BNC401050-500 1x 500 µL

CD3e (CRIS-7), CF640R conjugate, 0.1mg/mL

Sign In for Pricing
View Details
TGFalpha (TGFA/1119), CF594 conjugate, 0.1mg/mL
BNC941119-100 1x 100 µL

TGFalpha (TGFA/1119), CF594 conjugate, 0.1mg/mL

Sign In for Pricing
View Details
CD45 / LCA (135-4C5), CF640R conjugate, 0.1mg/mL
BNC400172-100 1x 100 µL

CD45 / LCA (135-4C5), CF640R conjugate, 0.1mg/mL

Sign In for Pricing
View Details