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MCL1 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

MCL1 apoptosis regulator, BCL2 family member

Gene Aliases

BCL2 family apoptosis regulator; bcl2-L-3; BCL2L3; bcl-2-like protein 3; bcl-2-related protein EAT/mcl1; EAT; induced myeloid leukemia cell differentiation protein Mcl-1; Mcl-1; MCL1, BCL2 family apoptosis regulator; mcl1/EAT; MCL1-ES; MCL1L; MCL1S; myeloid cell leukemia 1; myeloid cell leukemia ES; myeloid cell leukemia sequence 1 (BCL2-related) ; TM.

Gene ID

4170

Accession Number

NP_001184249.1

Reactivity

Homo sapiens, Human

Target

Induced myeloid leukemia cell differentiation protein Mcl-1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology MCL1 ELISA Kit (Human) (OKEH01489) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MCL1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MCL1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample MCL1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

1.56-100U/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 85%

Sensitivity

0.39U/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

37 kDa

Specificity

Natural and recombinant Human Induced myeloid leukemia cell differentiation protein Mcl-1

Protein Length

350

NCBI Gene Symbol

MCL1

Protein Name

Q07820

Gene Name URL

MCL1

Nucleotide Accession Number

NM_001197320.1

Frequently Asked Questions

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