LGALS13 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Galectin 13
Gene Aliases
Beta-galactoside-binding lectin; gal-13; GAL13; galactoside-binding soluble lectin 13; Galectin-13; lectin, galactoside-binding, soluble, 13; PLAC8; placental protein 13; placental tissue protein 13; PP13.
Gene ID
29124
Accession Number
NP_037400.1
Reactivity
Homo sapiens, Human
Target
Galactoside-binding soluble lectin 13
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology LGALS13 ELISA Kit (Human) (OKEH01488) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for LGALS13 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for LGALS13 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample LGALS13 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
15.6-1000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
Sensitivity
7.1 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
16 kDa
Specificity
Natural and recombinant Human Galactoside-binding soluble lectin 13
Protein Length
139
NCBI Gene Symbol
LGALS13
Protein Name
Q9UHV8
Gene Name URL
LGALS13
Nucleotide Accession Number
NM_013268.2
Frequently Asked Questions
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