UCP1 ELISA Kit (Human)
Product Specifications
Gene Name
Uncoupling protein 1
Gene Aliases
Mitochondrial brown fat uncoupling protein 1; SLC25A7; solute carrier family 25 member 7; thermogenin; UCP; uncoupling protein 1 (mitochondrial, proton carrier) .
Gene ID
7350
Accession Number
NP_068605.1
Reactivity
Homo sapiens, Human
Target
Mitochondrial brown fat uncoupling protein 1
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology UCP1 ELISA Kit (Human) (OKEH01465) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for UCP1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for UCP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample UCP1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
78-5000pg/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 100%
Sensitivity
28 pg/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
33 kDa
Specificity
Natural and recombinant Human Mitochondrial brown fat uncoupling protein 1
Protein Length
307
NCBI Gene Symbol
UCP1
Protein Name
P25874
Gene Name URL
UCP1
CAS Number
7732-18-5
Nucleotide Accession Number
NM_021833.4
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