NR1H4 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Nuclear receptor subfamily 1 group H member 4
Gene Aliases
BAR; bile acid receptor; farnesoid X nuclear receptor; farnesoid X-activated receptor; farnesol receptor HRR-1; FXR; HRR1; HRR-1; Nuclear receptor subfamily 1 group H member 4; PFIC5; retinoid X receptor-interacting protein 14; RIP14; RXR-interacting protein 14.
Gene ID
9971
Accession Number
NP_001193906.1
Reactivity
Homo sapiens, Human
Target
Bile acid receptor
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology NR1H4 ELISA Kit (Human) (OKEH01446) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for NR1H4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for NR1H4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample NR1H4 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
0.156-10ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 110%
Sensitivity
0.078 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
56 kDa
Specificity
Natural and recombinant Human Bile acid receptor
Protein Length
486
NCBI Gene Symbol
NR1H4
Protein Name
Q96RI1
Gene Name URL
NR1H4
Nucleotide Accession Number
NM_001206977.1
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