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IL4R ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Interleukin 4 receptor

Gene Aliases

CD124; IL-4 receptor subunit alpha; IL4R nirs variant 1; IL4RA; IL-4RA; interleukin 13 receptor; interleukin-4 receptor alpha chain; interleukin-4 receptor subunit alpha.

Gene ID

3566

Accession Number

NP_000409.1

Reactivity

Homo sapiens, Human

Target

Interleukin-4 receptor subunit alpha

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology IL4R ELISA Kit (Human) (OKEH01441) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IL4R has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IL4R is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample IL4R captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

15.6-1000pg/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 85%

Sensitivity

7.5 pg/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

90 kDa

Specificity

Natural and recombinant Human Interleukin-4 receptor subunit alpha

Protein Length

825

NCBI Gene Symbol

IL4R

Protein Name

P24394

Gene Name URL

IL4R

Nucleotide Accession Number

NM_000418.3

Frequently Asked Questions

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