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ALDH2 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Aldehyde dehydrogenase 2 family member

Gene Aliases

Acetaldehyde dehydrogenase 2; aldehyde dehydrogenase 2 family (mitochondrial) ; aldehyde dehydrogenase, mitochondrial; ALDH class 2; ALDH-E2; ALDHI; ALDM; epididymis secretory sperm binding protein; liver mitochondrial ALDH; nucleus-encoded mitochondrial aldehyde dehydrogenase 2.

Gene ID

217

Accession Number

NP_000681.2

Reactivity

Homo sapiens, Human

Target

Aldehyde dehydrogenase, mitochondrial

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology ALDH2 ELISA Kit (Human) (OKEH01438) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ALDH2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ALDH2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ALDH2 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

0.312-20ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%

Sensitivity

0.12 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

56 kDa

Specificity

Natural and recombinant Human Aldehyde dehydrogenase, mitochondrial

Protein Length

517

NCBI Gene Symbol

ALDH2

Protein Name

P05091

Gene Name URL

ALDH2

Nucleotide Accession Number

NM_000690.3

Frequently Asked Questions

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