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SLC2A4 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Solute carrier family 2 member 4

Gene Aliases

Glucose transporter type 4, insulin-responsive; GLUT4; GLUT-4; insulin-responsive glucose transporter type 4; solute carrier family 2 (facilitated glucose transporter), member 4; solute carrier family 2, facilitated glucose transporter member 4.

Gene ID

6517

Accession Number

NP_001033.1

Reactivity

Homo sapiens, Human

Target

Solute carrier family 2, facilitated glucose transporter member 4

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology SLC2A4 ELISA Kit (Human) (OKEH01437) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SLC2A4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SLC2A4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample SLC2A4 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 93%

Sensitivity

0.039 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

55 kDa

Specificity

Natural and recombinant Human Solute carrier family 2, facilitated glucose transporter member 4

Protein Length

509

NCBI Gene Symbol

SLC2A4

Protein Name

P14672

Gene Name URL

SLC2A4

Nucleotide Accession Number

NM_001042.2

Frequently Asked Questions

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