What is the catalog number for TNC ELISA Kit (Human)?

The catalog number for TNC ELISA Kit (Human) is OKEH01405-96W.

What sizes are available for TNC ELISA Kit (Human)?

TNC ELISA Kit (Human) is available in the following size(s): 96 Well.

Is TNC ELISA Kit (Human) in stock?

TNC ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

What is the recommended storage temperature after reconstitution?

Post-reconstitution storage guidance is listed on the datasheet, typically requiring aliquoting and storage at -20°C or -80°C to preserve activity.

Can this product be used in multiplex assays?

Multiplex compatibility depends on the specific product and detection panel. Contact our technical support team with details of your multiplex system for guidance before ordering.

What happens if this product doesn’t perform as expected in my experiment?

Send us the catalogue number, product name, lot number, and a summary of your results. Our technical support team will investigate and, where needed, involve the manufacturer. This is covered under our guarantee provided the product was used before its expiry date and stored correctly.

TNC ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Tenascin C

Gene Aliases

150-225; cytotactin; deafness, autosomal dominant 56; DFNA56; glioma-associated-extracellular matrix antigen; GMEM; GP; GP 150-225; Hexabrachion; hexabrachion (tenascin) ; HXB; JI; myotendinous antigen; neuronectin; tenascin; Tenascin-C; tenascin-C additional domain 1; TN; TN-C.

Gene ID

3371

Accession Number

NP_002151.2

Reactivity

Homo sapiens, Human

Target

Tenascin

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology TNC ELISA Kit (Human) (OKEH01405) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-human TNC antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated TNC and incubated. The TNC found in the sample or standards competes with the biotinylated TNC for limited binding sites on the immobilized anti-human TNC antibody. Excess unbound biotinylated TNC and sample or standard TNC is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated TNC captured in the well and inversely proportional to the amount of TNC which was contained in the sample or standard.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol