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PROM1 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

Prominin 1

Gene Aliases

AC133; antigen AC133; CD133; CORD12; hematopoietic stem cell antigen; hProminin; MCDR2; MSTP061; prominin-1; prominin-like protein 1; PROML1; RP41; STGD4.

Gene ID

8842

Accession Number

NP_001139319.1

Reactivity

Homo sapiens, Human

Target

Prominin-1

Type

ELISA Kit

Applications

Enzyme-linked Immunosorbent assay-Sandwich

Assay Principle

Aviva Systems Biology PROM1 ELISA Kit (Human) (OKEH01389) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PROM1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PROM1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample PROM1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids

Detection Range

0.156-10ng/mL

Recovery

Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 97%

Sensitivity

0.089 ng/mL

Reconstitution

Store as indicated in product manual.

Molecular Weight

97 kDa

Notes

Formerly GWB-04BZLP

Specificity

Natural and recombinant Human Prominin-1

Protein Length

865

NCBI Gene Symbol

PROM1

Protein Name

O43490

Gene Name URL

PROM1

Nucleotide Accession Number

NM_001145847.1

Frequently Asked Questions

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