OGDH ELISA Kit (Mouse)
Product Specifications
CAS Number
7732-18-5
Gene Name
Oxoglutarate (alpha-ketoglutarate) dehydrogenase (lipoamide)
Gene Aliases
2210403E04Rik;2210412K19Rik;2-oxoglutarate dehydrogenase complex component E1;2-oxoglutarate dehydrogenase, mitochondrial; AA409584; alpha-ketoglutarate dehydrogenase; d1401; mKIAA4192; OGDC-E1; oxoglutarate dehydrogenase (lipoamide) .
Gene ID
18293
Accession Number
NP_001239211.1
Reactivity
Mouse, Mus musculus
Target
2-oxoglutarate dehydrogenase, mitochondrial
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology OGDH ELISA Kit (Mouse) (OKEH01370) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for OGDH has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for OGDH is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample OGDH captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
0.78-50ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 87%
Sensitivity
0.401 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
116 kDa
Specificity
Natural and recombinant Mouse 2-oxoglutarate dehydrogenase, mitochondrial
Protein Length
1023
NCBI Gene Symbol
Ogdh
Protein Name
Q60597
Gene Name URL
Ogdh
Nucleotide Accession Number
NM_001252282.1
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