ERBB2 ELISA Kit (Rat)
Product Specifications
CAS Number
7732-18-5
Gene Name
Erb-b2 receptor tyrosine kinase 2
Gene Aliases
Epidermal growth factor receptor-related protein; p185erbB2; p185neu; Proto-oncogene c-ErbB-2; Proto-oncogene Neu.
Gene ID
24337
Accession Number
NP_058699.2Â
Reactivity
Rat, Rattus norvegicus
Target
Receptor tyrosine-protein kinase erbB-2
Type
ELISA Kit
Applications
Enzyme-linked Immunosorbent assay-Sandwich
Assay Principle
Aviva Systems Biology ERBB2 ELISA Kit (Rat) (OKEH01330) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ERBB2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ERBB2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ERBB2 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Urine, Tissue homogenates, Cell culture supernates, Other biological fluids
Detection Range
0.312-20ng/mL
Recovery
Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 102%
Sensitivity
0.165 ng/mL
Reconstitution
Store as indicated in product manual.
Molecular Weight
139 kDa
Specificity
Natural and recombinant Rat Receptor tyrosine-protein kinase erbB-2
Protein Length
1257
NCBI Gene Symbol
ERBB2
Protein Name
P06494
Gene Name URL
ERBB2
Nucleotide Accession Number
NM_017003.2
Curated Selection
Explore Other Products
Discover premium biology products from our extensive collection of 20M+ items
MBS2163973-01 0.1 mL
APC_Cy7-Linked Monoclonal Antibody to Cluster Of Differentiation 226 (CD226)
Ask
View Details MBS2163973-02 0.2 mL
APC_Cy7-Linked Monoclonal Antibody to Cluster Of Differentiation 226 (CD226)
Ask
View Details